1:11
Thank you very much, Darius, for that kind introduction.
1:13
Thank you, everyone, for attending this session.
1:17
I hope you find it beneficial.
1:22
Feel free to ask any questions or discuss new projects with us.
1:30
Today, I will provide an overview of some research tools we've developed to advance your projects in 3D culture and organoids.
1:42
Here's a brief overview of our company.
1:49
We have a founding molecule, TGF beta 1, which you see on the far left.
1:58
This molecule was our founding molecule in 1985, marking the mass production of the first commercially available cytokine.
2:05
Since then, we've expanded.
2:08
Depending on when you've interacted with our company, you might know us from different eras: R&B systems protein in the 1980s, simple instrumentation and nervous systems in the 1990s, small molecule range from Bristol in the 2000s, and RNA scope technology and 3D cell culture development in the 2010s.
2:34
We manufacture 90% of our reagents in-house, ensuring all are validated internally.
2:43
We are proud of our 600,000 citations in scientific journals.
2:54
Proteins are at our core, and we've just opened a state-of-the-art GMP facility at our headquarters.
3:07
This facility produces fully certified animal-free GMP proteins and handles all custom manufacturing for this range.
3:20
We aim to expand in the cell and gene therapy field, supporting projects from research to clinical phases.
3:30
One of our newest products is a pre-packed cytokine bag for use in clinical systems like Miltenyi prodigy.
3:47
Proteins form our base, and in recent years, we've moved into cell culture and cell biology.
4:06
We've seen many publications using our reagents in this field, prompting us to expand further.
4:15
At the core of our cell range are the BME/ECM products, with an entire range and ongoing development of the next generation animal-free synthetic products.
4:31
Our product development is driven by your needs, offering flexible solutions with thousands of small molecules, growth factors, and related reagents for your culture workflow.
4:57
We've been asked for protocols, and we've developed a central resource with recipes for common 3D cultures.
5:12
You can find protocols of interest on our website, making it easy to move into this field.
5:34
We're always open to developing new reagents for you.
5:42
We've heard from previous talks that different matrices are needed for various applications.
5:52
Our first-generation matrices were for 2D culture, then we moved into 3D culture and in vivo use.
6:14
Now, we're focusing on animal-free vitronectins and next-generation synthetic matrices.
6:27
The gold standard for matrices has been Matrigel, and we've developed a new matrix, UltiMatrix, based on your feedback. It took us 3.5 years to develop.
6:59
UltiMatrix is easy to use, with no need for batch testing and low endotoxin levels for co-cultures.
7:20
This matrix supports various organoids, including intestinal organoids for SARS-CoV-2 research.
7:32
Our Cultrex line was developed in consultation with Hynda Kleinman, the inventor of Matrigel.
8:06
The ideal matrix for stem cell-derived cultures and organoids has a high level of entactin, making a significant difference. These Western blots show a lot-to-lot consistency between 3 different proteins (laminin, collagen, and entactin) in the molecule.
8:32
We've shown consistency in key proteins and matrix stiffness using a rheometer.
9:09
Endotoxin levels are kept low to ensure consistency of cultures and to support complex cell systems and long-term 3D cultures.
10:00
Our matrix supports different types of organoids, including lung biopsy samples cultured for up to 52 days.
10:43
We've seen successful crypt formation in intestinal organoids and key markers in brain organoids.
11:11
Cardiomyocyte-derived organoids from iPSC cells are supported by this matrix.
11:49
Spheroids are another popular 3D culture system, and our matrix supports acinar maturation and consistency.
12:20
We've upscaled ECM production to meet global demand for 3D culture, with a facility in Atlanta dedicated to this.
12:55
Beyond ECMs, we offer a range of small molecules to support long-term culture vitality.
13:46
Non-invasive immunoassays and multi-analyte assays help investigate cell differentiation and maturation.
14:22
RNA scope is a highly specific method to detect RNA biomarkers in specific cells. It consists of Z target probes and you can pick your sequence of interest, we also have a signal amplification system and we have a detection and quantifications system.
15:00
We have probes for detecting both proteins and RNA, including a probe for LGR5, critical for stem cell research.
15:35
We've released our first animal component-free IPC expansion media, supporting reproducible results for up to 100 passages.
16:07
This media supports different IPSC lines with no difference in cell viability or genotype.
16:49
We can now differentiate IPSCs all the way to mature neurons completely animal and serum-free. The entire pathway is animal free.
17:24
We're excited to support researchers in this transition to animal-free culture.
18:36
In summary, we've shared key reagents and technologies for 3D culture workflow, including culture differentiation, isolation, verification, and analysis.
18:50
Our colleagues are available for more detailed discussions.
18:58
We have video protocols and resources to help in this field.
19:21
Please share your experiences with us, as your application of our products is the most interesting part.
19:39
Thank you for your attention.
