Kris Modig, Field Application Scientist at PipeBio discusses how tools like machine learning (ML) and bioinformatics can advance antibody discovery. PipeBio, a cloud-based bioinformatics platform, specializes in managing complex workflows for antibody and biologics discovery. PipeBio focuses on data challenges and offers innovative solutions to said challenges.
Key challenges in the field include managing large-scale, diverse data sets—ranging from sequence data to functional assay results—and addressing issues like data silos, error-prone methods (e.g., reliance on Excel), Furthermore, Modig highlights the challenge of heterogenous datasets, integration of diverse data types can lead to errors and misinterpretation. These challenges are exacerbated by the rapid increase in data generation from advanced sequencing techniques and public repositories.
Machine learning plays a pivotal role in overcoming these hurdles. It enables predictions for binding properties, developability parameters, and antibody stability. The platform integrates high-throughput data, such as antigen-binding datasets and structural data from tools like AlphaFold, to enhance model accuracy and efficiency. Highlighted tools include Sapiens for humanization predictions, NanoBodyToolkit for antibody engineering, and SaSa for developability assessments.
PipeBio's platform offers a centralised solution for data management, annotation, and visualisation. Users can process diverse data formats, validate sequence quality, and conduct lead optimisation using structural and functional predictions. The platform also supports workflows across various antibody types and custom scaffolds. By integrating diverse tools and maintaining a secure, reproducible database, PipeBio enables faster, more reliable analysis and collaboration within organisations.
Modig concludes with a discussion on machine learning applications, such as predicting antigen-binder properties in phage display, and emphasizes the importance of reproducibility, data visualization, and effective communication of results. Overall, PipeBio positions itself as a comprehensive, accessible solution for modern biologics discovery, fostering innovation and efficiency in the field.
